Journal: Journal of neurochemistry
Article Title: Pigment epithelium-derived factor (PEDF) and derived peptides promote survival and differentiation of photoreceptors and induce neurite-outgrowth in amacrine neurons
doi: 10.1111/jnc.15454
Figure Lengend Snippet: Immunocytochemistry data. Antibody combinations used in this paper, along with concentration and incubation time.
Article Snippet: N/A 1/400 Goat anti-rabbit (Alexa 488) Invitrogen A11001 PEDF-R (1) Sigma-Millipore ABD66 1/100 Goat anti-rabbit (Alexa 555) Invitrogen A27039 PEDF-R (2) Cayman 10006409 1/100 Goat anti-rabbit (Alexa 555) Invitrogen A27039 PEDF-R (3) R&D AF5365 1/100 Goat anti-mouse (Alexa 488) Invitrogen A28175 Rhodopsin Sigma-Millipore MAB5356 1/100 Goat anti-mouse (Alexa 488) Invitrogen A28175 Rhodopsin Gift of R. Molday N/A 1/100 Goat anti-mouse (Cy2) Jackson Immunoresearch 115–225-146 Tuj1 Abcam ab24610 1/300 Goat anti-mouse (Cy2) Jackson Immunoresearch 115-225-146 Acetylated Tubulin Abcam ab18207 1/300 Goat anti-rabbit (Cy3) Jackson Immunoresearch 115-16-003 Na/K pump Sigma-Millipore 06–520 1/100 Goat anti-rabbit/mouse (Alexa 555/488) Invitrogen A27039 /A28175 Open in a separate window * Primary antibodies were diluted in PBS and the incubation time and temperature were 1 h at 25°C or 16 h at 4°C.
Techniques: Immunocytochemistry, Concentration Assay, Incubation, CRAfT Assay